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stem loop primers  (Thermo Fisher)


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    Thermo Fisher stem loop primers
    Stem Loop Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stem+loop+primers/SUCROSE+EP%2FBP%2FNF+12KG/pm41816892-204-2-20
    Average 99 stars, based on 1 article reviews
    stem loop primers - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: MicroRNA-21 over expression in umbilical cord blood hematopoietic stem progenitor cells by leukemia microvesicles
    Article Snippet: Washed cells (without any microvesicles) were used for total RNA extraction by RNX Plus reagent (CinnaGen, Iran). .. Complementary DNAs (cDNAs) for microRNA-21 and Snord47 were then specifically synthesized according to the manufacturer’s instructions (ThermoFisher Scientific, Waltham, MA USA) using stem loop primers ( Mohammadi-Yeganeh et al. , 2013 ), as shown in . .. Quantitative real-time polymerase chain reaction (PCR) was performed to evaluate microRNA-21 gene expression fold change in an Applied Biosystems StepOne real-time system (Applied Biosystems, Foster City, CA, USA) using SYBR Green PCR master mix (TaKaRa, Japan) and specific primers ( ).

    Real-time Polymerase Chain Reaction:

    Article Title: Differential Expression of Defensins and Other Innate Immune Effectors by Indian Isolates of Chikungunya Virus.
    Article Snippet: Chikungunya virus (CHIKV) infection has been associated with heightened disease burden and fatality rate, especially post its resurgence in 2006, with presentation of various atypical symptoms.. Its re‐emergence and the subsequent periodic outbreaks indicate the constant evolution of the virus due to the incorporation of mutations in its genome.. This can potentially elicit a differential induction of innate immune response of varying degrees, which has been poorly studied in the case of CHIKV.

    SYBR Green Assay:

    Article Title: Differential Expression of Defensins and Other Innate Immune Effectors by Indian Isolates of Chikungunya Virus.
    Article Snippet: Chikungunya virus (CHIKV) infection has been associated with heightened disease burden and fatality rate, especially post its resurgence in 2006, with presentation of various atypical symptoms.. Its re‐emergence and the subsequent periodic outbreaks indicate the constant evolution of the virus due to the incorporation of mutations in its genome.. This can potentially elicit a differential induction of innate immune response of varying degrees, which has been poorly studied in the case of CHIKV.



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    miR-151-3p is a key functional cargo in Exe-Exos mediating anti-apoptotic and antioxidant effects. (A – B) Differentially expressed miRNAs between Exe-Exos and Sed-Exos groups (q < 0.05, |log 2 FC| > 1). (C) RT-qPCR validation of 10 differentially expressed miRNAs. (D) Flow cytometry analysis of apoptosis in OGD/R, OGD/R + Exos, and OGD/R + Exos + miR-151-3p groups. (E) Quantification of apoptotic cell percentage (n = 3 independent cell culture experiments). (F) LDH activity (U/L; n = 5 independent cell culture experiments). (G) WB and (H – M) quantification of p-JNK, JNK, C-Caspase9, Caspase9, C-Caspase3, and Caspase3 protein expression (n = 6 independent biological replicates). Exos exosomes, JNK c-Jun N-terminal kinase.

    Journal: Bioactive Materials

    Article Title: Exercise-derived exosomal miR-151-3p: An innovative anti-inflammatory and antioxidant therapeutic for spinal cord injury

    doi: 10.1016/j.bioactmat.2026.06.009

    Figure Lengend Snippet: miR-151-3p is a key functional cargo in Exe-Exos mediating anti-apoptotic and antioxidant effects. (A – B) Differentially expressed miRNAs between Exe-Exos and Sed-Exos groups (q < 0.05, |log 2 FC| > 1). (C) RT-qPCR validation of 10 differentially expressed miRNAs. (D) Flow cytometry analysis of apoptosis in OGD/R, OGD/R + Exos, and OGD/R + Exos + miR-151-3p groups. (E) Quantification of apoptotic cell percentage (n = 3 independent cell culture experiments). (F) LDH activity (U/L; n = 5 independent cell culture experiments). (G) WB and (H – M) quantification of p-JNK, JNK, C-Caspase9, Caspase9, C-Caspase3, and Caspase3 protein expression (n = 6 independent biological replicates). Exos exosomes, JNK c-Jun N-terminal kinase.

    Article Snippet: The miRNA stem-loop primers (Shanghai Jierui Bioengineering) are listed in .

    Techniques: Functional Assay, Quantitative RT-PCR, Biomarker Discovery, Flow Cytometry, Cell Culture, Activity Assay, Expressing